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H&E staining Mouse dorsal skin tissues were cut and soaked in 4% paraformaldehyde, placed at 4C for 48 h, dehydrated and embedded in paraffin, and sectioned to make tissue samples

Since the skin around the forehead, eyebrows, and eyes is significantly thinner than other areas of the face, selecting the correct depth is essential for both safety and effectiveness

Notably, the ILADS organization has also released a broader, more inclusive definition for CLD which encompasses those patients meeting the IDSA definition for PTLDS, as well as those with similar symptoms who would be excluded for lack of functional impairment or weaker evidence for prior Lyme disease (163)