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The recessed zone allows controlled airflow before smoke reaches the mouth, resulting in a smoother and cooler draw perception

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In brief, freshly prepared cell suspensions (10,000 cells per sample) were loaded into Chromium microfluidic chips with 3 (v3) chemistry and barcoded with a 10x Chromium Controller (10x Genomics)

Lifestyles variables Previous studies [17, 18, 33] have shown that unhealthy lifestyles such as alcohol abuse, video gaming addiction, and sleep deprivation are strongly associated with smoking or using e-cigarettes in young adults, so we added lifestyle variables to the study