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Flow cytometry (FACSCantoII, BD Biosciences, San Jose, USA) was used to analyze at least 10,000 PBMC per sample, and their population was defined by size and granularity ( Supplementary Figures 1AC )

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Suppressive effects of volatile anesthetics on PBMCs and macrophages have been demonstrated in in vitro studies

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The primary and secondary antibodies were used at the final titres of 1:2000 and 1:10,000, respectively

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