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The samples were then permeabilized in 0.5% Triton X-100 for 3 min and blocked with 3% goat serum albumin for 30 min prior to incubation with a primary antibody, namely, anti--synuclein, mouse anti-TH, SIGMAR1 Ab (Affinity Biosciences, Cat.#: DF7363) and anti-NeuN, at dilutions of 1:100 overnight at 4 C

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An association was also established between the A/A genotype of the rs7739889 PRL gene variant and reduced femoral neck BMD in the patient group (0.77 0.02) relative to the control group (1.12 0.13, p = 0.033)

Studies on the evaluation of the association between GPx1 and atherosclerosis variants in Japanese patients with type 2 diabetes, with four polymorphisms, reported that functional variants of the GPx1 gene are associated with increased mean intima-media (IMT) thickness of carotid arteries and cardiovascular risk and peripheral vascular disease in type 2 diabetics [63]