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Metabolomics APAP-induced liver injury biomarkers summary It is well established that translation of metabolic biomarkers from animal studies to humans is challenging due to the species difference as well as the confounding factors in humans including diet, age, lifestyle, overall health, drugdrug interaction and others, all of which will influence the metabolome profile

In vitro studies demonstrated cytotoxic activity in HepG2 cells and human liver cells L-02, leading to cell death, and demonstrated in in vivo studies that flavokavain B is responsible for the potentiation of kava hepatotoxicity upon herbdrug interaction in C57BL/6 mice