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Figure 3 3.5 Lipid storage Cells store excessive fatty acids in lipid droplets in the form of triglycerides (TAGs), which can serve as a protective reservoir against lipid oxidation stress (62)

Over the last two and half decades, many efforts have been made on developing and implanting in vitro protocols to successfully differentiate hPSCs into insulin-producing -cells with key features of bona fide mature -like cells using multi-stages directed differentiation protocols that recapitulate and phenocopy all specific stages of pancreas embryogenesis ( However, many protocols developed to differentiate stem cells into insulin-expressing -cells in vitro have faced a roadblock: the resulting -cells often exhibit an immature phenotype with impaired glucose-stimulated insulin secretion (GSIS) compared to cadaveric islets

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doi: 10.3389/fsufs.2024.1469635 225 UshaTMiddhaSKBhattacharyaMLokeshPGoyalAK