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Additionally, low b12 during early childhood has also been associated with higher risks of depression in adulthood (R)

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Protein samples were separated by 412% SDS-PAGE, transferred onto PVDF membranes (GE Healthcare, Milan, Italy), and incubated with antibodies targeting and Alpha-Smooth Muscle Actin (catalog n A2547, Sigma-Aldrich)

Failure of autophagy in this context promotes the accumulation of oxidative stress, aberrant signaling, mitochondrial damage, and chronic inflammation, collectively establishing a pro-tumorigenic cellular environment