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HFD differentially methylates vitamin D metabolism enzyme genes and VDR To identify the gene-specific methylation of CpG island of vitamin D metabolism genes and VDR, we adapted a digestion-based gene-specific PCR methylation assay

Unlike human brain tissue or co-culture systems, NGN2-induced neurons provide a homogeneous neuronal population, allowing direct assessment of metabolic remodeling to neurons themselves without confounding contributions from glial or vascular cells

Additionally, limited sample sizes and insufficient long-term follow-up in clinical trials impede comprehensive safety and efficacy assessments