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On observa que les patients souffrant danmie pernicieuse amlioraient leur tat avec la consommation de foie, puis avec ladministration dextraits concentrs issus de foies animaux, qui furent utiliss pendant des annes comme traitement de rfrence, jusqu ce quon identifie la vitamine B12 comme llment actif responsable de ces effets, ce qui permit de dvelopper sa synthse isole, ralise vers le milieu du XXe sicle

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Measurement of total serum IgG in huCD34+ engrafted NSG mice Serum was serially diluted in ELISA coating buffer in duplicate and incubated on 384-well microplates at 4 C for 16 h