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Our group [262] developed a multilayered defensive platform (IL-4@PEGRA NAs) with a size of 96.45 7.3 nm via the self-assembly of polycatechol-containing poly(ethylene glycol)-ylated phenolic rosmarinic acid (PEGRA) and IL-4, to preserve bioactivity and prolong circulation time

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Trypan blue for cell death and DAB staining for H 2 O 2 accumulation Detached leaves were immersed in trypan blue staining solution (2.5 mg ml 1 trypan blue dissolved in lactophenol:lactic acid:glycerol:liquid phenol:ddH 2 O at 1:1:1:1:1) for 30 min

Watanabe, J., Asaka, Y., and Kanamura, S