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As actual level of reaction product accumulated in the tissue section as a result of immunohistochemical reactivity is influenced by lots of factors, all parameters such as gray level adjustment, histogram stretch, and minimal optical density were carefully controlled following the recommended procedures

J Funct Foods 89:104946

One of the first chelating agents documented to reduce RS production (specifically the hydroxyl radical) in vitro was diethylenetriaminepentaacetic acid (DTPA)