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The supernatants of the cell lysates were isolated at 12,500 g for 30 min at 4 C and were incubated with anti-Flag magnetic beads overnight at 4 C, followed by extensive washing with NP-40 Lysis Buffer

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Persistent modification of the long half-life E3 ligase DCAF16, together with the relatively slow resynthesis rate of BRD9 in MV4-11 (8.6 h as determined by cycloheximide chase assay, Supplementary Fig