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The number of single or colocalized cells, the latter identified with overlapping TH + and EBF3 + staining, was performed manually by two independent investigators using the Cell Counter plugin in ImageJ

[DOI] [PubMed] [Google Scholar] 380.Chen, H

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18 During the maternal-to-zygotic transition, histone Kla, especially H3K18la, regulates the expression of zygotic genome activation (ZGA) genes such as Ccnt1 and Dppa2 , promoting embryonic development at the late G2 phase of the 2-cell stage in mouse embryos