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As shown by the docking poses, the positively charged Arg244 and Lys240 will electrostatically avoid each other and, therefore, might not be able to adopt the position required to exhibit the same effect as in the case of the respective single mutant (e.g

Farbood Y, Rashno M, Ghaderi S, Khoshnam SE, Sarkaki A, Rashidi K, et al