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On the basis of their performance in TR-FRET assays for the detection of Munc13-4STX7 binding and their inhibitory activity on TLR9 activation in cell-based assays, we selected ENDO3 and ENDO7 for initial downstream analysis and orthogonal validation

Cells were fixed and permeabilized (4% formaldehyde and 0.1% Triton-X 100/PBS 5 min at room temperature), followed by blocking (PBS, 0.1% Tween 20, 1% BSA, and 10% normal goat serum for 1 h or overnight at room temperature)

Sayori Wada, Email: [email protected]

Enzymatic activation by acyl-CoA synthetase long-chain family member 4 (ACSL4) and lysophosphatidylcholine acyltransferase 3 (LPCAT3) PUFAs cannot be directly peroxidized