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For Western blot analysis, the protein was transferred to Immobilon-P PVDF membrane (Merck Millipore, IPVH00010), which was then blocked with blocking buffer (Beyotime, P0252) for 1 h at room temperature

Mast cells contain an unusually high density of Ca 2+ release-activated Ca 2+ (CRAC) channels, which function to mediate mast cell activation

Proper hydration helps maintain mucosal lining integrity in the intestines and supports nutrient absorption

But Professor Nonhlanhla Khumalo, head of dermatology at Groote Schuur Hospital and the University of Cape Town, warned that it was unknown exactly how safe glutathione is