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The method, in generating a non-stochastic range of single amino acid substitutions, can comprise performing amplification reactions on the nucleic acid of step (a) using the set of oligonucleotides to generate a set of variant nucleic acids encoding nineteen amino acid substitution variants at a single amino acid residue of the antibody

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This transcriptional regulatory mechanism represents an important pathway for cells to enhance cystine uptake, promote glutathione synthesis, and thus respond to oxidative damage and maintain cellular redox homeostasis

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