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However, experiments using skin models generally underestimate absorption since the composition of model membranes does not reflect the structure of the stratum corneum, a heterogeneous, multi component membrane, nor does it account for the high hydrophilicity of GHK-Cu which may determine an idiosyncratic path of diffusion [10, 11]

Experiments on the biosynthesis of proteins from amino acids containing radioactive carbon or heavy hydrogen reveal that the protein molecule grows stepwise from the N terminus to the C terminus

For monolayer culture, MEFs and HC11 cells were seeded at a density of 1 10 4 cells/well (optimal cell-seeding density was optimized in preliminary experiments) in an XF96-well microplate 12 h before experiments

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