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After that, serial dilutions of the standards were made as follows: 100 L of 1000 M FeCl 2 solution into a 500 M FeCl 2 tube, 100 L from 500 M FeCl 2 solution into a 250 M FeCl 2 tube, 100 L from 250 M FeCl 2 solution into a 62.5 M FeCl 2 tube, and 100 L of a 62.5 M FeCl 2 tube into a 31.25 M FeCl 2 tube