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Sixteen hours after transfection, biotinylation was triggered by adding biotin to a final concentration of 50 M (1 mM biotin stock in PBS and stored for up to 6 months)

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Although there are limited published data on its potential benefits as a mucolytic agent in bronchiectasis (CF and non-CF bronchiectasis), our experience suggests it is being used in clinical practice in lung diseases associated with tenacious and copious sputum production, such as in allergic bronchopulmonary aspergillosis and bronchiectasis regardless of cause, and there is anecdotal evidence for the use of oral NAC as an antioxidant for IPF