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Limited clinical validation: Despite decades of research interest, DSIP has not progressed into wide-scale clinical adoption, which raises questions about consistency and reproducibility of effects

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Early synaptic dysfunction or synaptopathy at the level of inhibitory interneurons within the entorhinal cortex and CA3 hippocampal subregion leads to hyperactivity of pyramidal cells, which appears to play a role in the progression of AD neuropathology (e.g., tauopathy) (271, 272)

Each needle puncture through the rubber stopper introduces microscopic particles and dulls the needle tipalways use a fresh sterile syringe for each draw to maintain solution purity