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Furthermore, toll-like receptor (TLR)-mediated signals markedly increase Glut1 expression and glycolytic rates, which is detrimental to the stability and suppressive capacity of Treg cells [154]

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In the future, the integration of metabolomic readouts, single-cell and spatial transcriptomic localization, and metabolic modeling analysis is expected to establish a research framework of metabolic stratification-target selection-efficacy prediction, thereby reducing the risk of failed extrapolation across different diseases or populations (138)