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By prolonging the duration of SA -induced disease resistance in plants, the nanosystem enhanced various plant defense mechanisms, including reinforcement of physical barriers (lignin and suberin deposition), upregulation of antioxidant defense enzymes (POD, SOD, and CAT), the activation of defense-related genes ( OsPR1a , OsWRKY45 , OsAOS2 , and OsJAMYB ), and the stimulation of chitinase synthesis

Replacement of Asn450 by glutamine (N450Q), aspartate (N450D), and alanine (N450A) showed a significant increase in transpeptidase activity up to 3.6-fold enhancement and minor maturational defects, suggesting significant contribution of this residue in substrate binding and catalysis