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10.1016/0022-510x(96)00097-4 J

487 KingKEet al

Freshly smeared slides were air-dried for 20 min at room temperature and subsequently immersed in Carnoys solution (methanol/acetic acid 3:1) for 20 min to fix the hemolymph sample, then air-dried again (Pinheiro et al., 2013)