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Following clearing, nerves were mounted in SlowFade Gold Antifade Mountant with DAPI (Invitrogen, S36939) or without DAPI (Invitrogen, S36937)

doi: 10.3389/fphar.2025.1526270 Received 11 November 2024 Accepted 28 January 2025 Published 17 February 2025 Volume 16 - 2025 Edited by Alessandra Linardi, Santa Casa de So Paulo School of Medical Sciences, Brazil Reviewed by David Pubill, University of Barcelona, Spain Ariadiny Lima Caetano, Santa Casa de So Paulo School of Medical, Brazil Updates Copyright 2025 Wang, Hao, Ma, Feng, Yang, An, Su and Feng

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Once the displacement rinse is complete, close the mid-lateral outlet and proceed to the final rinse phasecommonly referred to as the "fast rinse." Introduce water from the top of the column and discharge it from the bottom, increasing the flow rate to 1520 m/h to perform a continuous rinse