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Conflict of interest MC has received consulting fees from AstraZeneca, Mallinckrodt Pharmaceuticals, MitogenDx, Werfen, Celltrion, Organon, and GlaxoSmithKline

doi: 10.1086/504691 390 SpitzerM.RobbinsN.WrightG

"Bacterial degradation of xenobiotic compounds: evolution and distribution of novel enzyme activities" (PDF)

Backcrossing is performed by reassembling (optionally in combination with other directed evolution methods described herein) the improved sequence with a large molar excess of the parental sequences