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Reduce reliance on supportive compounds after functional recovery improves and pain stabilizes below level 2

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of transferred embryos 1 1.0 1.0 2 0.86 (0.54, 1.38) 0.5306 0.79 (0.46, 1.38) 0.4119 Vitamin B12 Low 1.0 1.0 Average 1.44 (0.85, 2.44) 0.1790 1.83 (1.03, 3.25) 0.0409 High 0.88 (0.53, 1.48) 0.6396 1.05 (0.60, 1.83) 0.8600 Fig

Some common methods used for gene manipulation and their applications in Drosophila research are as follows: Mutant alleles LOF mutant alleles can be induced through various techniques, including chemical mutagenesis, radiation-induced mutagenesis, or more precise methodologies like CRISPR/Cas9 genome editing (Obe et al., 1971