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For GSH determination 30 minutes after APAP injection, liver tissue (50 mg) was homogenized with 20 volumes of 5% 5-Sulfosalicylic Acid Dihydrate (500 L) (Sigma-Aldrich) and centrifuged at 12,000 g for 10 minutes at 4 C

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When to See a Doctor While mild gas from B12 supplements is usually harmless, consult a doctor if you experience: Severe bloating or pain Diarrhoea or constipation Signs of an allergic reaction (rash, swelling, difficulty breathing) Final Thoughts Vitamin B12 itself doesnt directly cause gas, but certain supplement forms, additives, or high doses might contribute to bloating

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