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Introduction Coronavirus is a new global epidemic disease that first emerged in Wuhan, China in December 2019 and has since attracted worldwide interest

Western blot analysis Frozen liver samples were homogenized in RIPA buffer supplemented with protease inhibitors, left on ice for 10 min, and the supernatant was collected by centrifugation at 25.000 g for 10 min, as described by Li et al

This results in endoplasmic reticulum stress, lipid peroxidation, and ferroptosis, subsequently reducing TNF- and IFN- production and increasing PD-1 expression [139]

doi: 10.3389/fmed.2022.994911 153 HolvoetTJoossensMVzquez-CastellanosJFChristiaensEHeyerickLBoelensJet al