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The central executioners of apoptosis: caspases or mitochondria

We attribute this to a phenomenon we coined as reductive stress 51,92 where excessive antioxidant activity perturbs the delicate ROS balance required for normal sperm maturation 55,93

Briefly, cells were lysated in RIPA buffer (50 mM Tris, pH 7.5, 150 mM NaCl, 0.1% SDS, 1% Triton X-100, 0.5% deoxycholate and protease inhibitor cocktail (Roche)