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Fetisova E, Chernyak B, Korshunova G, Muntyan M, Skulachev V

Site-directed mutants E124 3.33 A, R283 6.55 A, F279 6.51 A, or W276 6.48 A almost abolished the activity of the ghrelin receptor, while F312 7.42 A impaired ghrelin-induced G-protein signaling, implying the critical role of the polar network and the WFF cluster in activation (Fig

And indeed, in cells lacking the glutathione reductase (Gor) ( gor ), Grx1-roGFP2 was around 60 % oxidized and neither addition of GSH, nor GSSG resulted in reduction of the probe (Figure 1E)