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Subsequently, IHC staining with anti-GFAP (glial fibrillary acidic protein) was also performed to visualize the intermediate filament (IF) protein expressed in numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells, with the number of GFAP-positive (GFAP + ) cells serving as an indicator for the activation of the neuroinflammatory pathway in the murine hippocampus (Fig

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