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Conjugated bile acids are more efficiently secreted into bile and are important for fat digestion, cholesterol disposal and the delivery of biliary compounds into the intestine

Luciferase and renilla signals were measured 48 h after transfection using the Dual Luciferase Reporter Assay Kit (Promega, Madison, WI, USA) according to a protocol provided by the manufacturer

Oxidative Medicine Cellular Longevity 2020, 9723686

While LPS exposure alone resulted in a 59% increase in oxidized DCF (a measure of ROS) compared to control cells, pretreatment with GHK-Cu at concentrations of 1, 5, and 10 M significantly decreased ROS production