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Calcium-dependent and calcium-independent activation of the endothelial NO synthase

Cells were incubated for 2 and 4 hours, after which the PBS was replaced with fresh medium and cytotoxicity was measured 24 hours later

The absorbance at 450 nm was measured using a Multiskan SkyHigh full-wavelength microplate reader (Thermo Scientific, A51119500C)

To gain insight into the response of LSC-Exo to SARS-CoV-2 infection at the gene level, we further evaluated a panel of genes involved in inflammatory responses in greater detail (Fig