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Moreover, it has been shown that gold nanoparticles under 15 nm were able to cross the BBB without any functionalization, probably through the transmembrane or the paracellular pathway, whereas gold particles bigger than 50 nm were not found in the brain [134-136]

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The recombinant FhGST-S1 enzyme was shown to be active, following the conjugation of substrates 1-chloro-2,4-dinitrobenzene (CDNB) and reduced glutathione according to an adaptation of the method described previously 25