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Gene expression profiling of 12633 genes in Alzheimer hippocampal CA1: transcription and neurotrophic factor down-regulation and up-regulation of apoptotic and pro-inflammatory signaling

TUNEL assay was performed using a DeadEnd fluometric TUNEL system (Promega, G3250) according to manufacturers instructions

General immune-related pathways included regulation of JNK cascade, inactivated in all 3 cell lines, and systemic lupus erythematosus, activated in DU145 and 22Rv1 and containing many complement, Fc receptor and HLA encoding genes (www.genome.jp/entry/pathway+hsa05322)