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READY TO STEP TO THE NEXT LEVEL

The supernatant was transferred to a fresh tube and incubated with the primary antibodies overnight on a rotator at 4C

hPSCs were dissociated into single-cell suspensions using TrypLE Select (Thermo Fisher Scientific, Netherlands) and plated at a density of 3.5 10 6 cells/well in E8 medium (Thermo Fisher Scientific, Netherlands) supplemented with 10 M Y-27632 (Peprotech, USA) on low-adhesion, non-treated 6-well plates (Eppendorf, Germany) placed on an orbital shaker at 37 C, 110 rpm, under a 5% CO 2 atmosphere