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Research published in the journal Sleep found that getting adequate sleep (at least seven hours) after drinking was linked to less severe hangovers

The cells were washed with PBS, fixed with 4% paraformaldehyde (MERCK # 30525-89-4) in PBS for 20 min at room temperature (RT), and subsequently permeabilized with PBS containing 0.5% Triton X-100 for 5 min