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TaqMan Reaction (Template Comes from RT Plate.) A master mix was prepared according to the following table: The primers used for the RT-PCR reaction is as follows: TaqMan Reactions (IkB Assays) Quantitative RT-PCR analysis was performed on total RNA preps that had been treated with DNaseI or poly A selected RNA

115,419 Under aerobic conditions, pyruvate is predominantly converted into acetyl-CoA in the mitochondria and further metabolized via the TCA cycle

This finding suggests a potential mechanism through which OIP5AS1 regulates glutaminolysis and influences melanoma tumor progression(Figure 2C) [185]