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FIGURE 9 Summary and conclusions: Glutathione and early (premature) inflammaging Chronic inflammatory diseases especially those compromising the lower respiratory system (chronic obstructive pulmonary disease, lower respiratory infections, cystic fibrosis, idiopathic pulmonary fibrosis, acute respiratory distress syndrome), diseases compromising the cardiovascular system (atherosclerosis, ischemic heart disease, stroke, and others), many other systemic inflammatory diseases like diabetes (274, 275)

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Primary neurons were plated in 24-well plates (1 10 5 cells/well) for 24 h, and the culture medium was replaced with conditioned medium from BV2 cultures for 24 h