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However, this rhythm was lost in NS nurses, indicating that NSW disrupts normal temporal regulation of estrogen receptor expression and may contribute to altered estrogen signaling 212

This is where affinity tags come in they are peptides which do not naturally exist in most expression systems that can be added to the N or C terminus of your protein of interest

Stable isotopic tracing of glutamine and GC-MS analysis U87-MG cells were seeded onto 35-mm dishes and incubated in growth medium until they reached confluence, washed thrice with warm HBSS, and incubated with modified HBSS (23 mM NaHCO 3 ) containing 1 mM of [ 13 C-5]l-glutamine (Cambridge Isotope Laboratories)