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7.4 Vision forward The future of PD care must be multidimensional, digitally enabled, and globally inclusive

They were then incubated with antibodies in dilution buffer (PBST with 0.5% FBS), 1:250 of anti-LC3B (Cell Signaling), and 1:500 anti-rabbit Alexa Fluor 488 (Cell Signaling) overnight at 4 C

After fixation, specimens were incubated with 30% sucrose for 2 h and frozen in isopentane previously kept in liquid nitrogen, as previously described 27

Proteins are necessary for collagen production and ECM deposition, with specific amino acids such as arginine aiding inflammation, collagen production, and macrophage function