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Pilot findings on SARS-coV-2 vaccine-induced pituitary diseases: A mini review from diagnosis to pathophysiology

Overall, this reaction of heteroaryl-nitriles with bis-thiols is shown to be highly efficient and versatile, of tunable reversibility, and offers enticing prospects as a new addition to the toolbox of biocompatible click-type reactions

NNMT (nicotinamide N-methyltransferase) is a cytosolic enzyme that catalyzes a specific methylation reaction: it transfers a methyl group from S-adenosylmethionine (SAM) to nicotinamide, producing two products 1-methylnicotinamide (1-MNA) and S-adenosylhomocysteine (SAH)

Then, the membranes were incubated overnight at 4 C with primary antibodies, followed by washing with Tris-buffered saline with Tween-20 (TBST)