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Fluorescence Resonance Energy Transfer (FRET)-based live-cell cAMP accumulation assay cAMP production mediated by Gs-coupled 2AR activation was measured using Exchange Protein Activated by cAMP 2 (Epac2) sensor containing a CFP and YFP FRET pair 31

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NSAIDS, including ibuprofen, aspirin, and celecoxib, all selectively antagonize the immune response by inhibiting the enzymes responsible for converting arachidonic acid into prostaglandins, cyclooxygenase 1 (COX-1) and cyclooxygenase 2 (COX-2)

Reprod Fertil Dev 13(78):459468 Hayakawa J, Joyal EG, Gildner JF, Washington KN, Phang OA, Uchida N, Hsieh MM, Tisdale JF (2010) 5% dimethyl sulfoxide (DMSO) and pentastarch improves cryopreservation of cord blood cells over 10% DMSO