Cardiolipin drives the catalytic activity of GPX4 on membranes: insights from the R152H mutant
All-optical Spatiotemporal mapping of ROS dynamics across mitochondrial microdomains in situ [J]
Table 26: the tabulation of the gene that when handling the HepG2 cell, in the diabetes array, is conditioned with 100 M Q10 Embodiment 13: about the PCR angiogenesis array of hepatocarcinoma (HEPG2) cell The thing that utilizes the medium processing HepG2 (hepatocarcinoma) cell carried out 24 hours or utilized 100 MQ10 to handle said cell and carry out different time.According to the method that is used for the PaCa2 cell (preceding text embodiment 9-11) with 1 * 10 5 Individual cells/well initiated process.Yet, be lower than expection from the total amount of the RNA of these sample extraction.Usually use total RNA (through what measure) of 1 g to carry out reverse transcription in the measurement at 260nm place.The spendable maximum volume of every reverse transcription is 8 L.Because RNA concentration is lower, so use utilize the medium thing and of the RNA of 0.44 g from 16 hours RT-PCR array analysis with the sample of 48 hours Q10 processing.Said array provides the trend and the preliminary analysis of pattern of the HepG2 Gene regulation of utilizing 100 M Q10 to handle to carry out, like what summarize in the following table 27.Through the different genes of finding when Q10 handles, to be conditioned be summarized in below in the table 27.Said result shows that among Gene A NGPTL3, ANGPTL4, CXCL1, CXCL3, CXCL5, ENG, MMP2 and the TIMP3 each was raised (reach respectively contrast about 5.5,3,3,3.2,3,3,1 and 6.5 times, 6 times and 5 times) on the 16th hour after processing.Handle the 16th hour ID3 in back at Q10 and be adjusted downward to about 1/5 of contrast.After processing the 48th hour
Age-based method The age-based method considers a childs age as the primary factor in determining dosage
