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[75] The Romans in the last form of the rite brought the sacrificial ox from Rome and every participant was bestowed a portion of the meat, a rite known as carnem petere

Then, UPLC-grade water DDW:MetOH (3:1, v/v) solution (0.5 mL) containing internal standards: 13 C- and 15 N-labeled amino acids standard mix (Sigma-Aldrich) was added to the tubes, followed by centrifugation