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Quantitative PCR (qPCR) for the measurement of gene expression was performed using the SYBR Green Master Mix on a CFX96TM Real-time system (Bio-Rad, United States)

Peptides need refrigeration because they are fragile molecules that break down over time, and cold dramatically slows that breakdown.

Long Half-Life of Both Components : Both components are acylated peptides with multi-day half-lives in humans, enabling research on sustained receptor signaling in relevant models