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For each 100 l of solvent A, 8.7 l of solvent B was then added and vortexed to neutralize the sample, and the mixture allowed to sit on dry ice for 20 min

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Three replications per treatment were used, and each replication comprised two pots

In recent years, intranasal delivery has attracted remarkable attention for brain targeting as the drug can be administered to the brain directly from the nose employing the trigeminal and olfactory pathways