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J Transl Med 21 , 300 (2023)

Cannabidiol, a Cannabis sativa constituent, as an antipsychotic drug

These studies suggest a close relationship between copper and necroptosis, indicating that copper can serve as a therapeutic target for inducing necroptosis in tumor cells and has potential application prospects in tumor treatment

Cell pellet was resuspended in cell lysis buffer (50 mM TrisHCl, 150 mM NaCl, 1% TritonX-100, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 2 mM EDTA and 50 mM sodium fluoride) containing 1 mM DTT, 1 mM PMSF and protease inhibitor cocktail