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[1] That acyl modification is not incidental chemistry: it is installed by a dedicated enzyme (ghrelin O-acyltransferase) and is required for the peptide to bind and activate GHSR1a

Also, a part of the left iliac vein was homogenized and lysed

HyPer7 has been previously shown to be highly selective for H 2 O 2 and sensitive enough to detect gradients of mitochondrial ROS in living organisms 11,12,31